Mirjana Đukić, PhD

Position: Full Profesor
Organizational unit: Department of Toxicology "Akademik Danilo Soldatović"
Address: Vojvode Stepe 450, 11221 Belgrade, Serbia
Room: A106
Phone: +381 113951 308
Fax: +381 11 3972 840
Email: mirjana.djukic@pharmacy.bg.ac.rs

CV

Bibliography


Employment Information

  • 2012-present, Full professor, Department of Toxicology, Faculty of Pharmacy, University of Belgrade
  • 2007-2012, Associate professor, Department of Toxicology, Faculty of Pharmacy, University of Belgrade
  • 2002-2007, Assistant professor, Department of Toxicology, Faculty of Pharmacy, University of Belgrade
  • 1988 -2002, Assistant, Department of Toxicology, Faculty of Pharmacy, University of Belgrade
  • 2007-present, Professor, Faculty of Pharmacy, University of Tuzla,  Bosnia and Herzegovina

 

Training and Education

Graduate and postgraduate studies:

  • 1984, BSc, Faculty of Pharmacy, University of Belgrade, Serbia
  • 1996, MSc (Toxicology): Defended master's thesis: Toxicological effects of disulfiram on the adrenergic system in alcoholics
  • 2001, PhD (Toxicology), Faculty of Pharmacy, University of Belgrade, Serbia: Defended doctoral thesis: Free radicals and antioxidant protection in the vulnerable brain structures of Wistar rats with acute poisoning with paraquat and diquat

Postdoctoral studies:

Research:

  • Oct 2002 – Oct 2003, Postdoctoral fellow at University of Pittsburgh, USA (Center for Free Radical and Antioxidant Health, lab of prof. Valerian E. Kagan, Department of Environmental and Occupational Health at the Graduate School of Public Health),
  • Nov 2010 - Nov 2011, Researcher Associate at University of Pittsburgh, USA (Center for Free Radical and Antioxidant Health, lab of prof. Valerian E. Kagan, Department of Environmental and Occupational Health at the Graduate School of Public Health)

Academic specialization:

  • 2015 – present, Biological drugs (an attendance), Faculty of Pharmacy, University of Belgrade, Serbia
  • 2015 – present, Risk Assessment (lecturer and attendance), Faculty of Pharmacy, University of Belgrade, Serbia

Courses:

  • 2004, „Introduction to Clinical Drug Research”, Vienna School of Clinical Research
  • 2005, „1st Balcan school for HPLC-MS“, Krug International Ltd., Hemtek d.o.o. and Waters Co.
  • 2005, „Promotion of the Three Rs (Reduction, Refinement, Replacement) Concept in Relation to Animal Experimentation”, JRC Enlargement & Integration Workshop, Alternatives, International Workshop on Croatia, Turkey and Western Balkan Countries, Ljubljana, Slovenia              
  • October 27, 2007, 4th Training course on “Concepts and Methods in Programmed Cell Death”, (ECDO) Portorož, Slovenia
  • September 6-9, 2008, 5thTraining course on 'Concepts and Methods in Programmed Cell Death' Bern, Switzerland

 

Teaching activity

Graduate Program:

  • 2002-present, lecturer, mentoring, co-author of students’ books

Mandatory courses:

  • Toxicology with analytics,
  • Clinico-toxicological analysis

Optional courses:

  • Free radicals and antioxidants –laboratory diagnostics (introduced and
  • organized by myself, 2008-present)
  • Drug abuse (introduced and organized by myself, 2008-present)
  • Acute drug poisoning
  • Ecotoxicology
  • Risk assesment

Postgraduate and Professional Programs:

Doctoral studies in Toxicology

  • 2002-present,: lecturer, mentoring (currently: 3PhD students). Titles of two doctoral theses:
    • The effect of disulfiram on the reproductive mechanisms of subacute toxicity of ethanol and / or cadmium in male rats  
    • Interference of thiol groups metabolite of disulfiram with glutathione response to oxidative stress induced by cadmium and / or alcohol in the rats liver)

Continuing education: lecturer and organizer     

  • 2005, Oxidative stress - clinical diagnostic significance,  
  • 2005, Prooxidant effects of xenobiotics and antioxidant protection
  • 2013, Drug abuse-Alcoholism

 

Scientific projects

  • 1996-2000, Biopharmaceutical and chemical technological investigations of drugs (Funded by The Ministry of Science, Serbia)
  • 2002-2005, Endogenous exposure parameters xenobiotics; analytics, monitoring, prophylaxis and treatmen (Funded by The Ministry of Science, Serbia)
  • 2002-2003, NIH-HD64145-01A1, Copper/albumin redox-cucling in preeclampsia (Funded by National Health Institute, USA)
  • 2002-2003, NIH-HL-70755, Pulmonary phosphatidylserine oxidation in apoptosis (Funded by National Health Institute, USA)
  • 2006-2010, Biochemical indicators of damage and dysfunction of organs (Funded by The Ministry of Science, Serbia)
  • 2008-2010, Women's health protection (Funded by The Ministry of Health, Serbia)
  • 2008-present, Clinical, pathophysiological and molecular aspects of trauma and inflammation (Funded by The Institute for Medical Research, Medical Military Acadaemy, Serbia)
  • 2009-2011, novel nitroxide resuscitation strategies in experimental traumatic brain injury (Funded by National Health Institute, USA, Co-Investigator, Valerian Kagan)
  • 2010-2011, Activation of cytochrome c into a peroxidase by esterified forms of vitamin E (Valerian Kagan, University of Pittsburgh, USA)
  • 2010-2011, Structure-based virtual screening to identify activators and inhibitors of peroxidase activity of cytochrome c (Valerian Kagan, University of Pittsburgh, USA)
  • 2010-present, Preventive, therapeutic and ethical approach in pre-clinical and clinical studies of genes and modulator redox cell signaling in the immune, inflammatory and proliferative response of cells (Ministry of Education and Science of the Republic of Serbia, III 41018)
  • 2012-present, The molecular mechanisms and potential modulation of oxidative cell state under physiological and pathophysiological conditions (Military Medical Academy, 3/12-14)

Research Interests:

Free radical toxicology, oxidative stress-related diseases and poisonings, bio-markers of diseases red-ox cycling compounds, the underlying mechanisms of drug/toxic agents toxicity and/or treatments, vitamin E analogues with antiproliferative effects, anticancer drugs, immune responses associated to oxidative/nitrosative stress, organ target toxicities. 

 

My team deals with

  1. Revealing the orchestration of glutathione cycle in hepatotoxicity induced by Cd and/or alcohol, as well as, optimization of  analytical HPLC-UV procedure for simultaneous determination of GSH and GSSG
  2. Understanding the Cd and alcohol reproductive and hepatotoxicity in rats and the effect of disulfiram (DSF) by assessing red-ox status and status of metals and relevant biochemical parameters. Therapy with DSF, has been intriguing to us, since the nucleophile character of its metabolite, DDTC.
  3. The effect of dietary prolonged zeolite intake  in rats on the status of lipid peroxidation in the brain (binding metal properties indirectly changed homeostasis of essential metals in the body and decreases MDA levels in the brain of the treated rats, probably by partial hindering the Fenton reaction)
  4. Understanding the paraquat and diquat neurotoxicity (model substances to induce overproduction of free radicals through red-ox cycling metabolism) through the use of various inhibitors: APV for NMDA receptors, naloxone for opiate receptors and L-NAME for NO-synthase. 
  5. Examination of oxidative stress parameters and immune response in gingival crevicular fluid (GSF) of the dental patients with a proximal caries treated with different dental restorative materials.
  6. Association of immune responses and oxidative stress (ROS, RNS, GSH, activities of arginase and NOS, antioxidant enzymes activities) in the serum of melanoma and dental patients, in different stages of disease.

 

Activities within wider Academic Community

Membership

  • 1999-present, Member of the Association of European Toxicologists and European Societies of Toxicology (EUROTOX)
  • 2010-present, Member of the Serbian Society of Toxicology  (SST)
  • 2006, Member of Editorial Board for National Encyclopedia Big home medical guide
  • 2007-2010, Member of Council of Faculty of Pharmacy, University of Belgrade
  • 2008-2011, Editor in Chief and Founder of official website for Faculty of Pharmacy, University of Belgrade

Reviewer

  • 2006-present, Acta Chromatographica
  • 2007-present, Food and Chemical Toxicology,
  • 2007-present, Basic of Clinical Pharmacology and Toxicology
  • 2007-present, Environmental Toxicology and Pharmacology
  • 2008-present, Free Radical Research
  • 2013-present, Bosnian Journal of Basic Medical Sciences
  • 2014-present, Vojno sanintetski pregled
  • 2014-present, Neurochemistry International
  • 2015-present Biological trace elements

Language: Serbian, English


  • Ervin Taso, Vladimir Stefanovic, Ivana Stevanovic, Danilo Vojvodic, Aleksandra Topic, Aleksandra Petkovic-Curcin, Kosovka Obradovic-Djuricic, Aleksa Markovic, Mirjana Djukic and Dragana Vujanović. Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid. Oxidative Medicine and Cellular Longevity Received 1 February 2018; Accepted 25 March 2018, Published 24 July 2018. 17 pages (IF 4.44)
  • Mirjana Djukic, Mara Fesatidou, Iakovos Xenikakis, Athina Geronikaki, Violina T. Angelova, Vladimir Savic, Marta Pasic, Branislav Krilovic, Dusan Djukic, Borko Gobeljic, Marina Pavlica, Ana Djuric, Ivan Stanojevic, Danilo Vojvodic, Luciano Saso. In vitro antioxidant activity of thiazolidinone derivatives of 1, 3-thiazole and 1, 3, 4-thiadiazole. Chemico-Biological Interactions, 25 April 2018; 286: 119-131. (IF 4.593)
  • Bisevac JP, Djukic M, Stanojevic I, Stevanovic I, Mijuskovic Z, Djuric A, Gobeljic B, Banovic T, Vojvodic D. Association Between Oxidative Stress and Melanoma Progression. Journal of Medical Biochemistry. 2018 Jan 1;37(1):12-20. (IF 1.148)
  • Djuric A, Begic A, Gobeljic B, Pantelic A, Zebic G, Stevanovic I, Djurdjevic D, Ninkovic M, Prokic V, Stanojevic I, Vojvodic D. Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes. Food and Chemical Toxicology. 2017 ;105:44-51. (IF 3.778)
  • Begic A, Djuric A, Ninkovic M, Stevanovic I, Djurdjevic D, Pavlovic M, Jelic K, Pantelic A, Zebic G, Saso L, Dejanovic B, Stanojevic I, Vojvodic D, Djukic M. Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium. Journal of Enzyme Inhibition and Medicinal Chemistry. 2017; 32(1):478-89. (IF 4.293)
  • Angelova VT, Valcheva V, Vassilev NG, Buyukliev R, Momekov G, Dimitrov I, Saso L, Djukic M, Shivachev B. Antimycobacterial activity of novel hydrazide-hydrazone derivatives with 2H-chromene and coumarin scaffold. Bioorganic & medicinal chemistry letters. 2017; 27(2), 223-227. (IF 2.930)
  • Stanojevic I, Miller K, Kandolf-Sekulovic L, Mijuskovic Z, Zolotarevski L, Jovic M, Gacevic M, Djukic M, Arsenijevic N, Danilo Vojvodic. A subpopulation that may correspond to granulocytic myeloid-derived suppressor cells reflects the clinical stage and progression of cutaneous melanoma. Int Immunol 2016; 28:87-97. (IF 3.748)
  • Angelova VA, Vassilev VNG, Nikolova-Mladenova B, Vitas J, Malbaša R, Momekov G, Djukic M, Saso L. Antiproliferative and Antioxidative effects of Novel Hydrazone Derivatives Bearing Coumarin and Chromene Moiety. Med Chem Res 2016; 25 (9): 2082–2092. (IF 1.277)
  • Djuric A, Begic A, Gobeljic B, Ninkovic M, Stanojevic I, Vojvodic D, Pantelic A, Zebic G, Prokic V, Dejanovic B, Stojanovic I, Pavlica M, Djukic D, Saso L, Djurdjevic D, Pavlovic M, Topic A, Vujanovic D, Stevnovic I, Djukic M. Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium. Food Chem Toxicol 2015; 86:25–33. (IF: 3.584)
  • Yanamala N, Kapralov AA, Djukic M, Peterson J, Mao G, Klein-Seetharaman J, Stoyanovsky DA, Stursa J, Neuzil J, Kagan VE. Structural re-arrangement and peroxidase activation of cytochrome c by anionic analogues of vitamin E: tocopherol-succinate and tocopherol-phosphate. J Biol Chem 2014; 289(47): 32488-98. (IF: 3. 43)